Fig. 3: The antigen presentation efficiency and performance mode of GDIII-Ferritin nanoparticle vaccine.
a Schematic representation of a fusion protein comprising multiple copies of Fagy-tagged GDIII antigens, an FTH1-1-based 24-meric nanoparticle, and a GDIII-Ferritin nanoparticle vaccine complex. b The states of ferritin and GDIII-Ferritin in solution were confirmed by analytical ultra-centrifugation (AUC) assay. The calculated molecular weights corresponding to each peak in AUC, where they are labeled above the curve. “Sed” stands for sedimentation. Experiments were conducted independently in triplicates. c Negative stain EM image of the GDIII-Ferritin nanoparticle vaccine. Raw image (left panels) and 2D classifications (right panels). The top left corner of the left panel is a representative image of particle picking. The green circles highlight representative vaccine particles. The 644 negative stain EM images of GDIII-Ferritin nanoparticle vaccine show similar antigen delivery efficiency and particle dispersion. d 3D reconstruction electron density map of the GDIII-Ferritin nanoparticle vaccine from negative stain EM data. The left panel shows a map of positive projection with threefold symmetry axes at the center. The right panel shows a map of positive projection with fourfold symmetry axes at the center. The entire map is displayed on an opaque surface, the delivery core is shaded in gray, and the spikes are colored in medium purple. e Fitting of the GDIII-Ferritin density map to the RABV GDIII structure (PDB: 6TOU) model and the Peptide 10-1/FTH1-1 structure model (PDB: 8WIE). The positions of GDIII, ferritin, and the Fagy tag are all indicated. The lower panel provides an enlarged view of the spike region of the GDIII-Ferritin density map fitted to the GDIII structure model. The map is displayed in transparent surface form.
