Fig. 2: The effect of electron dose on cryo-EM images of 6HBC RNA particles. | Nature Communications

Fig. 2: The effect of electron dose on cryo-EM images of 6HBC RNA particles.

From: Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography

Fig. 2

a A sample area was sequentially imaged with a dose of 50 e−Å−2 for 20 iterations. The images correspond to radiation damage with an accumulated dose of 50, 100, 150, and up to 1000 e−Å−2, respectively. b Zoomed-in images of 23 representative areas containing particles. c Fourier ring correlation (FRC) curves showing the effects of radiation damage, with each curve calculated by comparing the first image (50 e−Å−2) against the subsequent images. d A plot of FRC frequencies at FRC = 0.5 and 0.143, respectively, against their corresponding radiation doses.

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