Fig. 3: IPET 3D reconstruction and fitting model of an individual particle achieved at a dose of 168 e−Å−2. | Nature Communications

Fig. 3: IPET 3D reconstruction and fitting model of an individual particle achieved at a dose of 168 e−Å−2.

From: Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography

Fig. 3

a A schematic introducing the cryo-ET and 3D reconstruction methods. b The 3D reconstruction process of an individual particle by cryo-ET and IPET from a total electron dose of 168 e−Å−2, displayed from seven represented tilting directions. c The final 3D map viewed from two perpendicular directions. d Central slices of 3D reconstruction intermediate viewed from two perpendicular directions, comparing the stages before and after the application of soft masks and noise reduction during 3D reconstruction, and after missing-wedge (MW) correction and low-pass filtering to 8 Å. e Central slices of the final 3D map generated from two perpendicular directions. f The resolution measured by FSC curves of two half-maps reconstructed using even-odd frames (solid line) and tilt angle (dashed line) at FSC = 0.5 and 0.143, respectively. g The resolution estimated by FSC curves computed between the final map and its fitting model-generated map at FSC = 0.5. h The fitting model in the map is demonstrated by its central cross-section. i The 3D map with the fitting model viewed from 12 directions (rotated every 30° along the vertical axis).

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