Fig. 6: The effect of electron dose on IPET 3D reconstructions and their resolutions. | Nature Communications

Fig. 6: The effect of electron dose on IPET 3D reconstructions and their resolutions.

From: Non-averaged single-molecule tertiary structures reveal RNA self-folding through individual-particle cryo-electron tomography

Fig. 6

a Examples of IPET 3D reconstructions achieved at various total electron doses ranging from 54 to 597 e-Å-², with corresponding central slices shown (particles #10, 27, 67, 148, 165 from left to right panels). b The cross-sections of 3D reconstructions. c The 3D reconstructions. d The fitting models are viewed from two perpendicular directions, with rainbow-colored sequences indicating the order from the 5′ to 3′ end. e–g Plots of the resolutions of IPET 3D maps against their acquired electron doses. The resolutions are estimated by three different methods/criteria. The data for these plots is shown in Supp. Table 1.

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