Fig. 2: PATZ1 promotes adipocyte differentiation in vitro. | Nature Communications

Fig. 2: PATZ1 promotes adipocyte differentiation in vitro.

From: Transcription factor PATZ1 promotes adipogenesis by controlling promoter regulatory loci of adipogenic factors

Fig. 2

a Volcano plot of gene expression differences between PATZ1 and Vector cells. The log2FC of PATZ1/Vector was plotted as a function of −log10 (FDR) with selected genes indicated as upregulated in PATZ1 cells. b Heatmap showing upregulated, downregulated, and similarly expressed genes in Vector and PATZ1 cells (Left) and GO analysis of top genes upregulated and downregulated in PATZ1 cells (Right). Black arrows indicate “Fat cell differentiation”. c Heatmap showing top binned genes that are upregulated and downregulated in PATZ1 and Vector cells. d TF motif analysis 300 bp upstream of DEGs that are greater in PATZ1 cells. e Expression values of indicated genes represented as average FPKM values from PATZ1 or Vector cells. Vector: n = 3 and PATZ1: n = 3. f Oil-Red-O staining (Left) and qPCR analysis of indicated genes from control and PATZ1-overexpressing differentiated 3T3-L1 cells (Right). Vector: n = 4 and PATZ1: n = 4. Representative oil-Red-O staining from three independent experiments. g Bright-field microscopy (Left) and qPCR analysis (Right) of indicated genes from control and PATZ1 overexpressing human abdominal subcutaneous hADSCs differentiated for 12 days. Vector: n = 3 and PATZ1: n = 3. Representative microscopy images from three biological replicates. Scale bars, 100 μm. h (Right) Bright-field (top) and fluorescence at 509 nm (bottom) microscopy showing lipid droplets (top) and GFP fluorescence (bottom) in 7 days differentiated SVFs derived from PATZ1F/F mice infected with adenovirus (Adeno) expressing GFP or GFP-Cre and (Left) qPCR showing mRNA levels of indicated genes from PATZ1 F/F SVFs at D0 or D7 infected with Adeno GFP or Adeno GFP-Cre. GFP levels (bottom panel) show comparable viral infection. D0: n = 4, GFP: n = 4, and GFP-Cre: n = 4. Scale bars, 100 μm. i Domain structure of PATZ1 constructs with indicated deletion and qPCR showing indicated mRNA levels in D4 differentiated 3T3-L1 cells expressing indicated PATZ1 constructs. n = 4 per condition. e, g Results are from three independent experiments. e, g, h, i Unpaired Student’s t-test. Data were mean ± s.e.m. ***p < 0.001; **p < 0.01; *p < 0.05. e, g comparing PATZ1 to Vector; h comparing GFP-Cre to GFP; i all comparisons were made to PATZ1. Source data are provided as a Source Data file.

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