Fig. 4: Identification of the major adrenergic receptors.
From: Coordinated cellular behavior regulated by epinephrine neurotransmitters in the nerveless placozoa
A qPCR results showing the expression of potential adrenergic receptors in Trichoplax, N = 3 independent experiments. The graphs shown represent the mean ± SD of independent experimental replicates. B qPCR results show the expression of potential adrenergic receptors following shRNA knockdown. For each gene, the expression level of wild-type animals was set as 100%. NC, non-target control shRNA. WT, wild-type animals. N = 4 independent experiments. The graphs shown represent the mean ± SD of independent experimental replicates. two-tailed Unpaired t test. C–E Phenotypic analysis showing the motion behavior of the animals treated with Tad_60482 shRNA. Data are presented as mean ± SE, N = 3 independent experiments. Source data are provided as a Source Data file. F Unsupervised UMAP of scRNA-seq dataset (Accession number: 10.17632/bbpkbx968s.2) from Trichoplax adhaerens (H1) showing the cluster of different cell types as indicated. G Gene expression pattern of Tad_60482 on different cell types from scRNA-seq data. Relative expression level in different clusters is shown on the right. H FISH results show the expression of Tad_60482 in multiple cells. Scale bars, The same results were obtained in three independent experimental replicates. 100 μm in panel (C) and 20 μm in panel (H).
