Fig. 7: Two small GTPase Rab11A and Rab11B co-localize and interact with DHC3.

A Diagram showing the potential Rab protein as a cargo receptor linking dynein and cargo. B Eleven Rab genes in the Plasmodium and their transcript level in the gametocyte of P. berghei. Rab1A, Rab7, Rab11A, Rab11B, and Rab18 show relatively high transcript levels. The data comes from the published transcriptomes by Otto, T.D. 2014. C Co-localization analysis of DHC3 with Rab1A, Rab7, Rab11A, Rab11B, and Rab18 in ookinetes. The expression cassette of each rab gene was integrated into the p230p locus in the dhc3::6HA parasite for overexpression. Each gene was tagged with a 4Myc at the N-terminus and driven by the hsp70 5′-UTR and the dhfr 3′-UTR. Five double-tagged parasite lines were obtained and co-stained with antibodies against HA and Myc. Scale bars: 5 μm. Three independent experiments. D U-ExM of Rab11A and Rab11B association with SPMTs in ookinetes of two double-tagged parasite lines dhc3::6HA;4Myc::rab11a and dhc3::6HA;4Myc::rab11b. Parasites were co-stained with antibodies against α/β-Tubulin and Myc. Scale bars: 5 μm. Three independent experiments. E Myc-tagged Rab11A co-immunoprecipitated with the HA-tagged DHC3 in the dhc3::6HA;4Myc::rab11a (DTS1) ookinetes. The anti-Myc antibody was used for immunoprecipitation. BiP as the loading control. Three independent experiments. F Myc-tagged Rab11B co-immunoprecipitated with the HA-tagged DHC3 in the dhc3::6HA;4Myc::rab11b (DTS2) ookinetes. The anti-Myc antibody was used for immunoprecipitation. Three independent experiments. G HA-tagged DHC3 co-immunoprecipitated with the Myc-tagged Rab11B in the DTS2 ookinetes. The anti-HA antibody was used for immunoprecipitation. Three independent experiments. Source data are provided as a Source Data file.