Fig. 7: Normal basal synaptic transmission but impaired LTP in astrocytic NLG3 KO mice. | Nature Communications

Fig. 7: Normal basal synaptic transmission but impaired LTP in astrocytic NLG3 KO mice.

From: Astrocytic neuroligin 3 regulates social memory and synaptic plasticity through adenosine signaling in male mice

Fig. 7: Normal basal synaptic transmission but impaired LTP in astrocytic NLG3 KO mice.The alternative text for this image may have been generated using AI.

a Input/output curve of fEPSPs in GFAP-NLG3 KO (n = 18 slices, 8 mice) and GFAP-NLG3 WT mice (n = 13 slices, 6 mice). b Input/output curve of fEPSPs in NLG3 f/Y mice injected with GFAP-EGFP-Cre (n = 7 slices, 5 mice) or GFAP-EGFP virus (n = 8 slices, 5 mice). c Paired pulse ratio (PPF) in GFAP-NLG3 KO (n = 10 slices, 6 mice) and GFAP-NLG3 WT mice (n = 11 slices, 6 mice). d PPF in NLG3 f/Y mice injected with GFAP-EGFP-Cre (n = 9 slices, 5 mice) or GFAP-EGFP virus (n = 9 slices, 5 mice). e mEPSCs in GFAP-NLG3 KO (n = 9 cells, 3 mice) and GFAP-NLG3 WT mice (n = 10 cells, 3 mice). f AMPAR/NMDAR EPSC ratio in GFAP-NLG3 KO (n = 8 cells, 3 mice) and GFAP-NLG3 WT mice (n = 8 cells, 3 mice). g Impaired LTP in GFAP-NLG3 KO (n = 5 slices, 3 mice) compared to GFAP-NLG3 WT mice (n = 5 slices, 3 mice, p = 0.00000249). h Impaired LTP in NLG3 f/Y mice injected with GFAP-EGFP-Cre (n = 6 slices, 4 mice; p = 0.0175) compared to those injected with GFAP-EGFP virus. i Impaired dorsal hippocampal LTP in GFAP-NLG3 KO (n = 5 slices, 3 mice) compared to GFAP-NLG3 WT mice (n = 5 slices, 3 mice, p = 0.00505). j 50 nM TFB-TBOA (p = 0.009) restored LTP in GFAP-NLG3 KO mice whereas 150 nM TFB-TBOA did not (n = 5 slices, 3 mice for each group). Data represent mean ± SEM; two-tailed t test for (e, f, g, h, and i); repeated two-way ANOVA test with Holm-Sidak test for multiple comparisons for (c and d); One-way ANOVA for (j); *p < 0.05, *p < 0.01, ***p < 0.001. Scale bars: 0.5 mV/25 ms in (a and b), 30 pA/10 s in (e), 50 pA/50 ms in (f), 0.25 mV/50 ms in (g–j). Source data are provided as a Source Data file.

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