Fig. 5: ACC1 is a key downstream target regulated by circE7 in the epigenetic modulation of LGALS9 transcription.

Three ACC1 siRNAs were transfected into CAL27 and HN30 cells, or ACC1 was overexpressed in SCC090 and SCC154 cells. A RT-qPCR was used to detect the expression level of LGALS9 mRNA. B Western blot was used to detect the expression levels of ACC1 and galectin-9 proteins. Overexpression of circE7 in CAL27 and HN30 cells was accompanied by knockdown of ACC1, or knockdown of circE7 in SCC090 and SCC154 was accompanied by overexpression of ACC1. C RT-qPCR was used to detect the expression level of LGALS9 mRNA. D Western blot was used to detect the protein levels of ACC1, H3K27 Ac and galectin-9. Overexpression of circE7 in CAL27 and HN30 cells was accompanied by the addition of a small molecule inhibitor of ACC1, TOFA. E RT-qPCR was used to detect the expression level of LGALS9 mRNA. F Western blot was used to detect the protein levels of H3K27 Ac and galectin-9. Correlations were calculated using unpaired two-tailed Student’s t test for (A, C, E), *p < 0.05, **p < 0.01, and ***p < 0.001. These experiments were derived from three independent repetitions. Each western blots were reproduced three times with similar results (B, D, F). Data are presented as mean ± SD. Source data are provided as a Source Data File.