Fig. 6: MKC8866 synergizes with anti-PD-1 immunotherapy in syngeneic PCa mouse models.

A–C MKC8866 synergizes with anti-PD-1 treatment in Myc-CaP model. A Schematic representation of the experiment in the Myc-CaP mouse model. B Tumor volumes were measured at the indicated days for Vehicle + anti-IgG or Vehicle + anti-PD-1 (n = 4 mice, 7 tumors) and MKC8866 + anti-IgG or MKC8866 + anti-PD-1 (n = 4 mice, 8 tumors). C Same as in (B), but tumor weights were measured at the end of the experiment. D–F MKC8866 synergizes with anti-PD-1 treatment in Myc-CaP PTEN KO model. D Schematic representation of the experiment. E Tumor volumes measured at the indicated days are shown for Vehicle + anti-IgG (n = 6 mice, 11 tumors), Vehicle + anti-PD-1 (n = 8 mice, 15 tumors), MKC8866 + anti-IgG (n = 9 mice, 18 tumors), and MKC8866 + anti-PD-1 (n = 8 mice, 16 tumors). F Same as in (E) but tumor weights at the end of the experiment are presented. G–I MKC8866 enhances the efficacy of anti-PD-1 treatment in the RM-1 model. G Schematic representation of the experiment. H Tumor volumes measured at the indicated days are shown for Vehicle + anti-IgG (n = 6 mice, 11 tumors), Vehicle + anti-PD-1 (n = 7 mice, 14 tumors), MKC8866 + anti-IgG (n = 7 mice, 14 tumors), and MKC8866 + anti-PD-1 (n = 6 mice, 12 tumors). I Same as in (H), but tumor weights at the end of the experiment are presented. J Body weights of the mice from (B, E, and H) with the specified treatments for Myc-CaP, Myc-CaP PTEN KO, and RM-1 models respectively. Mean ± standard error by two-tailed student’s t test is presented for figure B, E, F, H, I, and J; unpaired two-tailed Mann Whitney t-test is used for C; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s, non-significant. Source data and exact p values are provided as a Source Data for figures (B, C, E, F, H, I, and J). Figures 6A, 6D and 6G were created in BioRender. Unal, B. (2023) BioRender.com/u75i711.