Fig. 3: Extending the biosynthetic pathway from (S)-NOR to produce (S)-RET. | Nature Communications

Fig. 3: Extending the biosynthetic pathway from (S)-NOR to produce (S)-RET.

From: De novo production of protoberberine and benzophenanthridine alkaloids through metabolic engineering of yeast

Fig. 3: Extending the biosynthetic pathway from (S)-NOR to produce (S)-RET.

a Schematic presentation of module II. P450 NMCH catalyzes the rate-limiting step of hydroxylation of N-methylcoclaurine. CPR could shuttle two electrons to P450 for its catalyzation. Optimal coupling of alternative P450 and CPR enables improved activity. (S)-RET titers and final OD600 in engineered strains carrying b Various combinations of NMCH and CPR, and c Multiple copies of rate-limiting enzymes. Significance was calculated using two-tailed t-test. Data are presented as mean ± standard deviations (n = 4 biologically independent samples). Source data are provided as a Source Data file.

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