Fig. 6: In vivo determination of resting and dynamic inhibitory receptor driving forces in neurons.
From: All-optical reporting of inhibitory receptor driving force in the nervous system
a Schematic of the experimental setup (top) and a widefield fluorescence image of a region of L1 in S1 with GAD2+ interneurons expressing ORCHID (bottom), with similar images obtained in each mouse (n = 10 mice); scale bar: 100 µm. b LFP recordings were used to independently record population activity (top, black traces). ORCHID was used to concurrently record DFGABAA in GAD2+ interneurons in L1 of the anaesthetised mouse brain (bottom, orange traces), where a variety of different DFGABAA values were recorded, including hyperpolarizing (left), purely shunting (centre), and depolarising DFGABAA (right). Inset: widefield fluorescence images of the cells from which recordings were made; scale bars: 10 µm. c Population data showing DFGABAA in L1 interneurons in S1 (0.3873 mV ± 0.5348 mV, n = 123 cells from 10 mice). d 4-AP (500 µM) was infused into the cortex buffer in the recording chamber over the craniotomy to investigate DFGABAA dynamics during SLEs in vivo. ORCHID recording from a GAD2+ interneuron before (left) and after (right) a 4-AP induced SLE (bottom, orange traces). An LFP recording provided a readout of network activity (black traces, purple bar indicates SLE). e Population data of DFGABAA in GAD2+ interneurons showed a large depolarising shift in DFGABAA post-SLE (paired t test, two-tailed P = 0.000033, n = 22 cells from 4 mice). -DFGABAA values plotted. ****P ≤ 0.0001; error bars indicate mean ± SEM.
