Fig. 1: Modification of the Tle phospholipase domain. | Nature Communications

Fig. 1: Modification of the Tle phospholipase domain.

From: Advanced glycation end-product crosslinking activates a type VI secretion system phospholipase effector protein

Fig. 1

a Tle and Tle-Tli production was induced with arabinose in E. cloacae and E. coli cells. Urea-soluble total protein was extracted for immunoblot analysis using anti-Tle polyclonal antisera. Molecular mass markers (kDa) are on the left. This experiment was performed independently three times with similar results. b His6-lipase domains were produced with (or without) ∆ss-Tli in E. coli cells, then purified by Ni2+-affinity chromatography under non-denaturing (native) or denaturing conditions for SDS-PAGE analysis. Molecular mass markers (kDa) are on the left. c Deconvoluted mass spectrum of purified modified His6-lipase domain. d In vitro phospholipase A1 activities of purified lipase domains. e In vitro phospholipase A2 activities of purified lipase domains. Phospholipase activity data are presented as mean values ± standard deviation for three independent experiments. Source data are provided as a Source Data file.

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