Fig. 4: Aldehyde reductases suppress Tle crosslinking. | Nature Communications

Fig. 4: Aldehyde reductases suppress Tle crosslinking.

From: Advanced glycation end-product crosslinking activates a type VI secretion system phospholipase effector protein

Fig. 4

a His6-Tle was produced with Tli in E. coli cells that overexpress the indicated methylglyoxal detoxification enzymes. His6-Tle was purified by Ni2+-affinity chromatography under denaturing conditions and analyzed by SDS-PAGE. Molecular mass markers (kDa) are on the left. b Competition co-cultures. E. cloacae inhibitor strains that overexpress methylglyoxal detoxification enzymes were co-cultured with E. cloacae ∆tle-tli target bacteria and viable cells enumerated as described in Methods. Competitive indices are averages ± standard error for three independent experiments. Source data are provided as a Source Data file.

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