Fig. 1: ZIKV RNA synthesis and translation.
From: Zika viruses encode 5′ upstream open reading frames affecting infection of human brain cells
A Western blot analysis of ZIKV E protein and GAPDH in Vero and U251 cells infected with American isolate PE243 and African isolate Dak84 (MOI:3) for 24 h. GAPDH was used as a loading control. Molecular masses (kDa) are indicated on the left. Infections were performed in triplicate with similar results. B Map of the 10,807-nt ZIKV/Brazil/PE243/2015 genome. The 5′ and 3′ UTRs are in black, and the polyprotein ORF is in pale blue with subdivisions showing mature cleavage products. Histograms show the read densities, in reads per million mapped reads (RPM), of Ribo-Seq (red) and RNA-Seq (green truncated at 100 RPM for better visualisation) reads at 24 h p.i. (repeat 1) in Vero cells pre-treated with CHX. The positions of the 5′ ends of reads are plotted with a +12 nt offset to map (for RPFs) approximate P-site positions. Negative-sense reads are shown in dark blue below the horizontal axis. In light blue, the translational efficiency (TE) is calculated as the positive-sense Ribo-Seq/RNA-Seq ratio. Source data are provided as a Source Data file.
