Fig. 1: Schema for energy transfer.
From: Supercharged fluorescent proteins detect lanthanides via direct antennae signaling
a Schema of the posited lanthanide-based resonance energy transfer between the lanthanide antenna and the fluorescent protein (PDB: 2B3P). Antenna excitation gives rise to a detectable signal if the lanthanide and the biosensor are separated by 10–100 Ă…. Created in BioRender. Huang, K. (2023) BioRender.com/f23r502. b Spectral overlaps between terbium, thulium, dysprosium, and YFP, GFP, and CFP. The solid dashes represent select free-ion energy levels of trivalent terbium, thulium, and dysprosium. The vertical bars denote the excitation maxima +/− the spectral half-width of supercharged YFP, GFP and CFP. Source data are provided as a Source Data file.
