Fig. 3: Time-resolved spectroscopy reveals long-lived lanthanide luminescence signaling.
From: Supercharged fluorescent proteins detect lanthanides via direct antennae signaling
a Supernegative GFP−10 sensors fluoresce in the presence of 1 mM of Tb3+. Time-resolved luminescence responses measured at 0 µs, 100 µs, and 300 µs. GFP−10 concentration of 0.1 mg/mL (~3.7 µM) in 50 mM Tris-HCl at pH of 7. Green shading denotes the fraction of GFP−10 fluorescence attributable to Tb3+ placement. b Supernegative YFP−31 sensors fluoresce in the presence of 1 mM of Tb3+. Time-resolved luminescence responses measured at 0 µs, 100 µs, and 300 µs. YFP−31 concentration of 0.1 mg/mL (~3.7 µM) in 50 mM Tris-HCl at pH of 7. Yellow shading denotes the fraction of YFP−31 fluorescence attributable to Tb3+ placement. Source data are provided as a Source Data file.
