Fig. 4: Measures of biofluorescence emission in amphibians satisfies two of Marshall and Johnsen’s criteria for ecological relevance of biofluorescence.
From: Evidence for ecological tuning of anuran biofluorescent signals

A Criterion 2 (fluorescence will be viewed against a contrasting background): wavelength of peak emission in anurans (green circles) tends to be different than the most abundant wavelengths in background twilight (p < 0.0001; black line digitized with permission from Cronin et al., 2014). B Criterion 3 (organisms viewing the fluorescence will have spectral sensitivity in the fluorescent emission range): peak emission wavelengths (green circles) match peak sensitivity of anuran green-sensitive (GS) rod of the anuran visual system better than expected by chance (p < 0.0001; black line obtained from34). In each panel, the observed wavelength of emission for each individual frog is presented as a colored circle (n = 194). The mean irradiance and sensitivity values of the environment and GS rod at each emission wavelength was not directly measured in this study but obtained from irradiance/sensitivity spectra. Randomization tests were used to generate null distributions and test for significance (see text for details).