Fig. 6: Coarse-grained ensembles of eIF4B IDR suggest a direct role of the DRYG-rich region in driving oligomer formation.
From: Disordered regions of human eIF4B orchestrate a dynamic self-association landscape
a, b Experimental (black) and simulated (red and blue) mean transfer efficiencies, \(\left\langle E\right\rangle\) as a function of labeled positions for monomeric DRYG-CTR (a) and CTR (b) at an ionic strength of I = 192 mM (corresponding to buffer ionic strength of 20 mM NaP with 150 mM NaCl). c Correlations between the experimental and simulated \(\left\langle E\right\rangle\). The concordance correlation coefficients (ρc) are reported in the legend for DRYG-CTR and CTR, respectively. d Experimental (black) and simulated (orange) \(\left\langle E\right\rangle\) as a function of labeled positions for DRYG-CTR simulated at an ionic strength of I = 117 mM (corresponding to buffer ionic strength of 20 mM NaP with 75 mM NaCl) and conditions favoring formation of oligomers (see “Methods” for details). e Correlation between the experimental and simulated \(\left\langle E\right\rangle\), with the concordance correlation coefficients (ρc) reported in the legend. f Difference in the fraction of contacts between monomeric and oligomeric DRYG-CTR, at an ionic strength of I = 117 mM, as observed in experiments. Darker regions indicate the intermolecular contacts when DRYG-CTR is in its oligomeric state, with an increase in inter-chain contacts particularly seen within the DRYG region (residues 213–331). g Bar plot showing the size of oligomers obtained from the simulations of DRYG-CTR in the oligomeric state at I = 117 mM. Each oligomer size is expressed as a percentage of the simulated trajectory and normalized considering the size of the smallest oligomer (2-mer). h Representative conformations of oligomeric clusters of eIF4B IDR. The DRYG-rich region is represented as gold, whilst the CTR is shown in gray. Source data are provided as a Source Data file.
