Fig. 2: B. acidifaciens is enriched in DSS-treated mice transplanted with the fecal microbiota of synbiotic-supplemented mice. | Nature Communications

Fig. 2: B. acidifaciens is enriched in DSS-treated mice transplanted with the fecal microbiota of synbiotic-supplemented mice.

From: Galactooligosaccharides and Limosilactobacillus reuteri synergistically alleviate gut inflammation and barrier dysfunction by enriching Bacteroides acidifaciens for pentadecanoic acid biosynthesis

Fig. 2

After a week of acclimation, the intestinal microbiota of 4-week-old male C57BL/6 J mice (n = 8 per group) were depleted with a cocktail of antibiotics for four weeks, followed by two-week daily gavage of PBS or the fecal microbiota prepared from the mice fed standard chow (CONFMT) or GOS/L. reuteri (GLFMT). Colitis was induced in mice by providing 3% DSS in the final week. a, b Study design and body weight change in DSS-treated mice with/without treatment with CONFMT or GLFMT. c–e The length, H&E staining, Alcian blue staining, histological damage score of the colon of DSS-treated mice with or without treatment with CONFMT or GLFMT. f, g Serum concentrations of pro-inflammatory cytokines and relative protein expression levels of tight junction proteins in the colon of DSS-treated mice with/without treatment with CONFMT or GLFMT. h–k Principal coordinates analysis (PCoA) of weighted UniFrac distances, microbiota composition, and differential abundance of the fecal microbiota at the genus and specie levels as determined by RT-qPCR. Samples for western blot were derived from the same experiment and gels/blots were processed in parallel. One-way ANOVA with Tukey’s test was used for statistical analysis of body weight and intestinal parameters, and the Kruskal-Wallis test and post-hoc Dunn’s test was used for microbial analysis. Data were presented as mean ± SEM. Source data were provided as a Source Data file.

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