Fig. 4: The H3/H4 tetramer was deposited on the replicated strands by the Mcm2 N-tail in the simulations of the replicated-DNA-engaged replisome with zero or one RPA.

Source data are provided as a Source Data file. A Schematic illustration of the initial structures. The color scheme is the same as in Fig. 1B. B Initial structures with zero (left) and one (right) RPA molecule. C The number of residues in the H3/H4 tetramer contacting the lagging strand (pink), the leading strand (cyan), and Cdc45 (yellow) in the case of zero (left) and one (right) RPA molecule. D Ratios of the replicated strands on which the H3/H4 tetramer was deposited in the simulations with zero, one, and two RPA molecules. E The same as in (D), but trajectories were classified into the Cdc45-mediated and unmediated pathways. F Schematic illustration explaining the definition of the 3D distance from the fork junction to the H3/H4 tetramer. G Distances from the fork junction to the H3/H4 tetramer at the moment of recycling to the lagging strand via the Cdc45-mediated pathway (orange; n = 4, 8, and 4) and the Cdc45-unmediated pathway (gray; n = 13, 12, and 3) in the simulations with zero, one, and two RPA molecules. The error bars represent the mean ± standard deviation. H Schematic illustration explaining the definition of the distance from the fork junction to the destination location. I Distances from the fork junction to the position of the tetrasome dyad on the lagging (pink; n = 17, 20, and 7) and leading strand (cyan; n = 11, 18, and 13) in the simulations with zero, one, and two RPA molecules. The error bars represent the mean ± standard deviation.