Fig. 5: The H3/H4 tetramer was deposited on the daughter strands by the Mcm2 N-tail in the simulations of the replicated-DNA-engaged replisome without Pol ε and RPA.

Source data are provided as a Source Data file. A The structure of CMG (opaque) superimposed to that of CMGE (CMG + Pol ε) (transparent). B, C 1D and 2D probability distributions of the leading strand orientation calculated from the simulations of CMGE and CMG. D Ratios of the replicated strands on which the H3/H4 tetramer was deposited in the simulations of CMGE and CMG. E, F 2D distributions of the H3/H4 tetramer orientation calculated from trajectories of the simulations of CMGE and CMG (G) Distances from the fork junction to the deposited positions on the lagging (pink; n = 17 and 24) and leading strand (cyan; n = 11 and 13) in the simulations of CMGE and CMG. The error bars represent the mean ± standard deviation. H, I Schematic illustrations and representative structures of CMGE (H) and CMG (I). The initial structure and the structure at the exact moment of the H3/H4 deposition are shown on the left and right, respectively. The color scheme is the same as in Fig. 1B, D. Additionally, the green and magenta spheres on the leading strand represent the average recycled position in the CMGE and CMG simulations, respectively.