Fig. 5: Mutational effects can be decomposed into their general and specific components.
From: The genetic architecture of protein interaction affinity and specificity
a, b Specifcity plots representing the binding scores as a function of the global binding energy (ΔΔG) inferred by MoCHI. The sigmoid curve represents binding scores predicted by MoCHI, and the vertical residual from the curve thus represent the specific effect of this mutation on the interaction with this partner. The three characters in the mutant name correspond to heptad, position within the heptad and amino acid substitution, respectively. Mutant names are colored according to their heptad position, which further highlights that several mutations at the same few positions have specific effects (a, black; b, red; c, green; d, blue; e, cyan; f, pink; g, yellow). Examples with the four partners presented in Fig. 2B (a) and general principle of the decomposition of specific and global effects (i.e., effects due to change in global energy) (b) (WT = wilde-type). c distribution of mutational effects due to changes in global binding energy (top) and specificity (bottom). Plot of mutational effects as a function of their specificity (d) and global binding energy (e) components. Each point represents a different variant-partner pair.
