Fig. 4: Analysis of evolutionary patterns.
From: Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell
Heatmap of mutation frequencies enriched to at least 5% at any round of in vitro evolution. Int-WT(1), trial 1 of intermittent in-vesiculo evolution on a starting WT (codon-optimized) sequence of mod-ori-p2p3. Int-WT(2), trial 2 of intermittent in-vesiculo evolution on a starting WT (codon-optimized) sequence of mod-ori-p2p3. Int-Mut, intermittent in-vesiculo evolution on a starting F62Y variant of codon-optimized sequence mod-ori-p2p3. Con-WT, semi-continuous in-vesiculo evolution on a starting WT (codon-optimized) sequence mod-ori-p2p3. And, Bulk-WT, serial transfer of bulk IVTTR reaction starting with WT (codon-optimized) sequence mod-ori-p2p3.
