Fig. 5: Immunomodulatory property and mechanism of the BNP-PEDOT-PSF-AG hydrogel in vitro. | Nature Communications

Fig. 5: Immunomodulatory property and mechanism of the BNP-PEDOT-PSF-AG hydrogel in vitro.

From: Polyphenol-mediated redox-active hydrogel with H2S gaseous-bioelectric coupling for periodontal bone healing in diabetes

Fig. 5

a Immunofluorescence staining of THP-1 cells on the different hydrogels. iNOS (red) stained M1 macrophages. CD163 (green) stained M2 macrophages. b Polarization of macrophages on the different hydrogels were evaluated by expression of CD86 (M1) and CD206 (M2) using flow cytometry. GSEA analysis of lipid catabolic process and lipid biosynthetic process (c) and glutathione peroxidase activity (d). e Heatmap of genes involved in macrophage polarization, lipid catabolic process, lipid biosynthetic process, and glutathione peroxidase activity. f Schematic illustration of the mechanism of immunomodulatory ability of BNP-PEDOT-PSF-AG hydrogel on THP-1. The blue arrow showed the effect of the BNP-PEDOT-PSF-AG hydrogel. Gpx glutathione peroxidase, FA fatty acid, PUFA polyunsaturated fatty acids.

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