Fig. 5: Mechanisms of bacterial resistance development to rumicidins.
From: Rumicidins are a family of mammalian host-defense peptides plugging the 70S ribosome exit tunnel
a MICs of rumicidin-1 against obtained rumicidin-1-resistant E. coli strains (R1 and R2) and other strains in the Mueller-Hinton broth supplemented with 0.9% NaCl. * - the lptD gene bears the deletion of 23 codons (Asp330-Asp352) which makes the outer membrane of the strain more permeable to antibiotics20. b Resazurin visualization of the antimicrobial microdilution test performed for rumicidin-1 against BW25113 ΔrpoS constructively expressing the additional allele of either macB or the mutant macBN470D. Viable cells reduce blue resazurin to pink resorufin. c The proposed mechanism of action of rumicidins against E. coli.
