Fig. 5: GmNRAMP2a&2b are responsible for the Fe transfer for SNF, as well as the inhibition of SNF by inorganic N.

a–d Comparison of seedling (a, b) and nodule growth (c, d) in WT and nramp2ab mutants under non-symbiotic or symbiotic conditions. 4-day-old seedlings were grown under non-symbiotic (non-inoculation with high-N (H–N) supply) or symbiotic (inoculation with low-N (CK) supply) conditions for 22 d. e–j Comparison of ureide export rate (e), nodule Fe intensity (f, g), Fe bodies in uninfected cell (h, i), lytic vacuoles in infected cell (j) in WT and nramp2ab mutants. Nodules at 22 dpi were treated with or without H–N (CK) for 2 d. Nodule samples were sectioned and stained with Perls solution, followed by DAB intensification. Yellow arrows and blue asterisks in (h) indicate Fe bodies and lytic vacuoles, respectively. k–n Comparison of ureide export rate (k), nodule Fe intensity (l), Fe bodies in uninfected cell (m), lytic vacuoles in infected cell (n) in overexpression lines and their segregated WT. Nodules at 22 dpi were treated with or without H–N (CK) for 2 d. Nodule samples were sectioned and stained with Perls solution, followed by DAB intensification. The boxes in (b, d, e, g, i–n) indicate the first and third quartiles, and the whiskers indicate the minimum and maximum values. The lines within the boxes indicate the median values. n = 6 (b, d, k), 5 (e), 10 (g, l), 50 (i, m), or 20 (j, n) biologically independent replicates. Scale bars = 15 cm (a), 1 mm (c), 50 μm (f, h). Values were calculated using two-sided t-test. The phenotypic statistics for the overexpression lines were based on two independent lines, and for each treatment, at least three plants per line were evaluated. Source data are provided as a Source Data file.