Fig. 2: Cassette identity dictates expression and the resistance level of downstream ARCs.
From: The expression of integron arrays is shaped by the translation rate of cassettes
a GFP fluorescence of all 136 pMBA-ARC strains measured by flow cytometry normalized to fluorescence of pMBA control strain. Bars represent the mean and SEM of n = 3 biological replicates. Red bars denote ARCs with non-significant differences. Statistical significance was assessed using a one-way ANOVA followed by Dunnett’s multiple comparison test (fluorescence of each ARC vs fluorescence of pMBA). p-values for all ARCs are listed in Supplementary Data 2). b Distribution of the GFP fluorescence ratios (pMBA-ARC/pMBA) with indication of the mean value and SD, n = 136 cassettes. c Violin plot depicting cassette length distribution of all 136 ARCs. Median and quartiles are represented. d Correlation between relative GFP fluorescence levels and ARC length. r indicates Pearson’s correlation coefficient e Relative fluorescence of aacA54, aacA61 and aacA8 cassettes. Bars represent the mean and SD of n = 5 biological replicates. Statistically significant differences were determined using two-sided unpaired t-test, ***P < 0.001; Resistance levels conferred by pMBAØ or pMBA carrying the indicated arrays to cefaclor, ertapenem and chloramphenicol. Bars depict the mean and SD of the MIC values of n = 3 independent biological replicates. A red dotted line indicates the clinical breakpoint (EUCAST) for E. coli against the respective antibiotic. Source data are provided as a Source Data file.
