Fig. 4: Reduced IFN-γ production in Hif1α− / − T cells is not due to proliferative defect.
A, B Naïve WT and Hif1α− / − CD4+ T cells were activated under normoxia (21% O2) and hypoxia (1% O2) for 5 days. Gated live cells were analyzed for the expression of ICOS and CD25 (A), depicted as geometric mean fluorescence intensity (gMFI), and area of forward scatter (FSC-A) (B) (N = 4 per group, ****p < 0.0001, ***p = 0.0001). C, D Naïve WT and Hif1α− / − CD4+ T cells were labeled with CellTrace Violet (CTV) and activated under normoxia (21% O2) and hypoxia (1% O2). CTV dilution was monitored daily to assess cell proliferation (C). IFN-γ production by activated WT and Hif1α− / − CD4+ T cells within indicated cell divisions was shown (D) (N = 3 per group, ****p < 0.0001). Two-way ANOVA with Šídák’s multiple comparisons test (with adjustment) was used for (A, B and D). All the experiments were repeated at least twice. Pooled results shown in the dot plots and bar graphs depicted means ± SEM for all the samples in each group, with each dot denoting an independent sample. Source data were provided in the Source Data file.
