Fig. 1: Spatial single-cell morphometrics highlights cell orientation, stress direction and size as the main parameters defining apical cell morphology in the dorsal pericardial wall.

a Workflow for the single-cell morphometric analysis of cells in the dorsal pericardial wall. b, c Description of the cell features used for the single-cell morphometrics. d Heatmap showing the coefficients for each feature that compose each joint spatial principal component axis (jsPCA). e Heatmap showing the eigenvalues for each principal component. f Visualization of the main joint spatial PCA1 parameters: cell orientation and stress tensor in real space. g Visualization of the main jsPCA2 parameters: cell area and cell perimeter in real space. A anterior, P posterior, R right, L left, λ stress amplitude, ϑ stress main orientation, e embryo, JSPC Joint spatial principal component. N = 6 embryos, n = 8618 cells. Source data are provided as a Source Data file.