Fig. 3: Spatial single-cell morphometrics reveals that patterned tension in the second heart field emerges during heart tube formation.

a, b. Scheme of the architecture of the SHF at embryonic days 8.5 (a) and 9.5 (b) showing breakdown of the dorsal mesocardium (DM) between these timepoints. c Frontal view of E8.5 wild-type whole mount dorsal pericardial wall, main cell orientation map, stress map and cluster map. d Frontal view of E9.5 wild-type whole-mount dorsal pericardial wall, main cell orientation map, stress map and cluster map. Scale bars: 100 µm. NT neural tube, AP arterial pole, VP venous pole, r right, l left, d′ dorsal, v ventral, a anterior, p posterior. Wild-type E8.5: N = 6 embryos, n = 2994 cells; wild-type E9.5: N = 6 embryos, n = 8618 cells.