Fig. 4: Clusters defined by spatial single-cell morphometrics correlate with the distribution of T-box transcription factors in the dorsal pericardial wall. | Nature Communications

Fig. 4: Clusters defined by spatial single-cell morphometrics correlate with the distribution of T-box transcription factors in the dorsal pericardial wall.

From: Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field

Fig. 4

a–e Frontal whole-mount views of the DPW after RNAscope fluorescent in situ hybridization showing Tbx1 (green) and Tbx5 (magenta) mRNA distribution between E8 (6 somites) and E9.5 (25 somites), before and after dorsal mesocardial breakdown. Nuclei are stained using Hoescht (blue) (f) Summary of the expression profiles of Tbx1 and Tbx5 based on the schemas in Fig. 3a, b, showing the links between Tbx1 and Tbx5 expression domains and clusters 1–3, and the input of retinoic acid signaling14. AP arterial pole, VP venous pole, aSHF anterior second heart field, pSHF posterior second heart field. E8-E8.5: N = 5 embryos, E9: N = 3 embryos, E9.5: N = 6 embryos. Scale bars: 100 µm.

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