Fig. 5: T-box transcription factors TBX1 and TBX5 pattern apical cell architecture in the dorsal pericardial wall. | Nature Communications

Fig. 5: T-box transcription factors TBX1 and TBX5 pattern apical cell architecture in the dorsal pericardial wall.

From: Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field

Fig. 5

a–c Ventral whole-mount views of E9.5 wild-type, Tbx1-/-, and Tbx5 conditional mutant DPWs stained with phalloidin. a′–c′. Cell segmentation of E9.5 wild-type, Tbx1 mutant, and Tbx5 conditional mutant and cells labeled according to their cluster number (cluster 1 green, cluster 2 orange, cluster 3 purple). d Boxplot quantifications of the spatial coordinates of cells belonging to each of the clusters along the antero-posterior axis (with y coordinates normalized from -1 (VP) to 1 (AP)) for E8.5 (p = 0.96, p = 1) and E9.5 wild-type (***p = 5.89e-35, ***p = 5.91e-64), Tbx1-/- (***p = 5.00e-29, ***p = 1.08e-52) and Mef2cAHFCre;Tbx5fl/fl embryos (p = 0.93, p = 0.81); p-values are based on Mann Whitney U test and test whether the spatial distribution of clusters 1 or 2 is more anterior than that of cluster 3. Centre lines show the median, box limits show the first and third quartiles, and whiskers show maximum and minimum values. For statistical tests, the following biological replicas were analyzed: wild-type E8.5: N = 6 embryos, n = 2994 cells; wild-type E9.5: N = 6 embryos, n = 8618 cells; Tbx1-/-: N = 3 embryos, n = 2551 cells; Mef2cAHFCre;Tbx5fl/fl: N = 4 embryos, n = 2809 cells. Scale bars: 50 µm.

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