Fig. 6: TBX5 induces stress anisotropy and orients cells parallel to the antero-posterior axis in the posterior dorsal pericardial wall. | Nature Communications

Fig. 6: TBX5 induces stress anisotropy and orients cells parallel to the antero-posterior axis in the posterior dorsal pericardial wall.

From: Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field

Fig. 6: TBX5 induces stress anisotropy and orients cells parallel to the antero-posterior axis in the posterior dorsal pericardial wall.The alternative text for this image may have been generated using AI.

a–d Whole-mount immunofluorescence of the DPW. Phalloidin in white, TBX5 in red and nuclei in blue. a–d Ventral views of the DPW of wild-type embryos at E8.5 (a) and E9.5 (b) compared to Tbx1-/- (c) and Mef2cAHFCre;Tbx5fl/fl (d) mutant embryos, showing from left to right: whole-mount immunofluorescence of the DPW (phalloidin in white, TBX5 in red, nuclei in blue), cell orientation map, spatial plot of the cells whose main orientation angle is 90° ± 10 compared with left-right axis of the embryo on the segmentation mask of the apical cell membrane, cell stress map, cell anisotropy map and morphometric map. e Boxplot quantification of the spatial coordinate of cells oriented vertically (90° ± 10) or horizontally (0/180°) along the anterior-posterior axis (with y coordinates normalized from -1 (VP) to 1 (AP)); p-values are calculated using a Wilcoxon signed-rank test. For horizontal cells: E9.5 wild-type, ***p = 1.69e-27, Tbx1-/-, ***p = 1.17e-18, Mef2cAHFCre;Tbx5fl/fl, p = 0.02. For vertical cells: E9.5 WT: ***p = 1.25e-39, Tbx1-/-: ***p = 5.11e-27, Mef2cAHFCre;Tbx5fl/fl: ***p = 0.96. f Boxplot quantification of cells with anisotropic stress (<0.3) along the antero-posterior axis (with y coordinates normalized from −1 (VP) to 1 (AP)). p-values are calculated using a Wilcoxon signed-rank test and test whether the spatial distribution of cells deviates from a symmetric distribution. Error bars represent standard deviation. In boxplots in panels e and f, centre lines show the median, box limits show the first and third quartiles, and whiskers show maximum and minimum values. E9.5 wild-type, ***p = 5.45e-41, Tbx1-/-, ***p = 4.34e-5, Mef2cCreAHF;Tbx5fl/fl, p = 0.10. For statistical tests, the following biological replicas were analyzed: wild-type E8.5: N = 6 embryos, n = 2994 cells; wild-type E9.5: N = 6 embryos, n = 8618 cells; Tbx1-/-: N = 3 embryos, n = 2551; Mef2cAHFCre;Tbx5fl/fl: N = 4 embryos, n = 2809 cells. Scale bars: 50 µm.

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