Fig. 3: Fibrinogen is largely trapped in the inclusion bodies of SEL1L-HRD1 ERAD-deficient hepatocytes.
From: Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD
Fibrinogen staining using a rabbit anti-serum recognizing all chains of fibrinogen in liver sections from: (a) WT and Sel1LAlb littermates at different ages; (b) Sel1Lf/f mice at different time points after an i.v. injection of AAV8 expressing TBG-driven Cre or GFP; (c) Hrd1f/f mice 4 weeks after an i.v. injection of AAV8-TBG-Cre or GFP; (d) WT mice 4 weeks after i.p. injections of vehicle (Veh) or cyclopiazonic acid (CPA) at 5 mg/kg body weight every other day; (e) Ire1αf/f mice 6 weeks after an i.v. injection of AAV8-TBG-Cre or GFP. Insets of higher magnification shown below. Arrowheads point to apical and sinusoidal localization of fibrinogen; arrows point to inclusion bodies containing fibrinogen. Representative data from n = 3 mice per cohort.
