Fig. 5: Increased synthesis of 18:1 phospholipids accelerates growth recovery from cold shock in respiration-dependent conditions. | Nature Communications

Fig. 5: Increased synthesis of 18:1 phospholipids accelerates growth recovery from cold shock in respiration-dependent conditions.

From: A temperature-sensitive metabolic valve and a transcriptional feedback loop drive rapid homeoviscous adaptation in Escherichia coli

Fig. 5: Increased synthesis of 18:1 phospholipids accelerates growth recovery from cold shock in respiration-dependent conditions.

Growth monitored in a 6-well plate by optical density (symbols). Derivatives of optical density measurements (smoothed over 7 points) yields instantaneous growth rates (lines); each line represents an independent culture. A Comparison of wild-type (WT), ΔfabR, and ΔfabF growth in defined media containing indicated carbon sources at indicated temperatures (0.2% w/vol glucose + 0.1% w/vol CAS amino acids, 0.2% glycerol, or 0.2% succinate). The number of independent cultures is indicated for each experiment. B Comparison of wild-type and ΔfabF (one experiment for each condition) following cold shock from 37 °C to 0 °C and subsequent recovery at 26 °C in a microplate reader with tergitol carrier (control), palmitate (16:0 fatty acid), or cis-vaccenate (18:1 fatty acid). Source data are provided as a Source Data file.

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