Fig. 1: HURP contains different elements that can activate or decelerate Kif18A motility.
From: HURP regulates Kif18A recruitment and activity to synergistically control microtubule dynamics
a Domain organization of full-length HURP and Kif18A, and different HURP and Kif18A truncations used in this study (NL: neck-linker). b Schematic of the in vitro reconstitution of Kif18A motility on surface-immobilized microtubules (MT) in the presence of HURP44. c Representative fluorescence images showing microtubule binding of HURP constructs used for motility assays. All HURP constructs were tested at 1 µM concentration. d (Left) Representative images showing HURP1-285 binding to microtubules at different concentrations. (Right) Quantification of HURP1-285 binding to microtubules. The center circle and whiskers represent the mean and standard deviation (S.D.), respectively. Kd was determined from a fit to a Hill equation (dashed curve, N = 20 microtubules for each condition). e Representative kymographs showing motility of 2 nM Kif18A in the presence of 1 µM of different HURP constructs. Microtubule and HURP signals are shown in green and yellow, respectively. Representative kymographs are from the same microscopy session, with the same batches of proteins. f Normalized run frequency (N = 20 kymographs for each condition), velocity, and run time (N = 50 motors for each condition) of 2 nM Kif18A in the presence of different HURP constructs. The center line and whiskers represent the mean and S.D., respectively. P values were calculated from a two-tailed t-test, compared to the no HURP condition. The in vitro motility assays were performed with 3 technical replicates. Source data are provided as a Source Data file.
