Fig. 6: Role of the IMVGI variant rs734209466 in enhancing IGFBP2 and IGFBP5 transcription. | Nature Communications

Fig. 6: Role of the IMVGI variant rs734209466 in enhancing IGFBP2 and IGFBP5 transcription.

From: Integrative 3D genomics with multi-omics analysis and functional validation of genetic regulatory mechanisms of abdominal fat deposition in chickens

Fig. 6: Role of the IMVGI variant rs734209466 in enhancing IGFBP2 and IGFBP5 transcription.

a Integrative Genomics Viewer (IGV) plot illustrating the regulatory role of candidate functional variants in AFD traits, as identified by IMVGI analysis. b Sanger sequencing validation results displaying the sequencing curves for SNP rs16596562 and InDel rs734209466, confirming the presence of these variants in the selected broiler chicken population. c Violin plot depicting phenotypic differences in AFD traits (AFW and AFP) among 330 NEAUHLF individuals with various genotypes (n = 136 for rs16596562-GG, n = 86 for rs16596562-GA, n = 108 for rs16596562-AA, n = 131 for rs734209466-DelDel, n = 87 for rs734209466-InDel and n = 112 for rs734209466-InIn, biologically independent animals). P values were obtained using one-way ANOVA followed by Tukey’s multiple comparisons test. d Luciferase–based enhancer assay results demonstrating the enhancer activity of different alleles in the chicken preadipocyte cell line (ICP2). e RT‒qPCR assessment of the mRNA expression levels of candidate target genes of IMVGI variants in the FL and LL of abdominal adipose tissue. P values were obtained using unpaired two-tailed Student’s t-test. f, g Luciferase–based promoter assays were used to evaluate the transcriptional effects of enhancer alleles of the two IMVGI variants on the promoter regions of their target genes. d, f, g P values were obtained using one-way ANOVA followed by Dunnett’s multiple comparisons test. d, e, f, g Data were presented as mean ± SD, n = 9 biologically independent samples. Source data are provided as a Source Data file.

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