Fig. 7: The transcription factor IRF4 mediates differential allelic activity of rs734209466. | Nature Communications

Fig. 7: The transcription factor IRF4 mediates differential allelic activity of rs734209466.

From: Integrative 3D genomics with multi-omics analysis and functional validation of genetic regulatory mechanisms of abdominal fat deposition in chickens

Fig. 7

a The results of the competitive EMSA of ICP2. The binding affinity of the protein to DNA oligos was demonstrated by adding unlabeled rs734209466–Del or unlabeled rs734209466–In to the reaction. b rs734209466–In resides within the IRF4 binding motif predicted by both the Homer and AnimalTFDB4 databases. c, d Luciferase–based enhancer assays were performed to detect changes in enhancer activity before and after the overexpression of IRF4 in ICP2. Data were shown as the mean ± SD, n = 9 biologically independent samples (unpaired two-tailed Student’s t-test). e IRF4 supershift EMSA of ICP2. 1×, 2× and 4× represent 1 μg, 2 μg and 4 μg of HA antibody, respectively. f RT‒qPCR analysis was utilized to detect the expression of IGFBP2 and IGFBP5 rs734209466–InIn genotyped preadipocytes, with and without the overexpression of IRF4. Data were shown as the mean ± SD, n = 6 biologically independent samples (unpaired two-tailed Student’s t-test). Source data are provided as a Source Data file.

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