Fig. 8: Effects of silencing and overexpression of IGFBP2 and IGFBP5 on fat deposition: preadipocyte proliferation and differentiation. | Nature Communications

Fig. 8: Effects of silencing and overexpression of IGFBP2 and IGFBP5 on fat deposition: preadipocyte proliferation and differentiation.

From: Integrative 3D genomics with multi-omics analysis and functional validation of genetic regulatory mechanisms of abdominal fat deposition in chickens

Fig. 8: Effects of silencing and overexpression of IGFBP2 and IGFBP5 on fat deposition: preadipocyte proliferation and differentiation.

a Cell viability was analyzed using the CCK–8 assay. P values were obtained using two-way ANOVA followed by Dunnett’s multiple comparisons test, n  =  6 biologically independent samples. b The mRNA levels of the preadipocyte proliferation markers PCNA and Ki67 were assessed by RT‒qPCR (n = 6 biologically independent samples). c, d Assessment of DNA synthesis in cells using EdU staining (scale bars: 500 μm, n = 3 biologically independent samples). e, f The distribution of the cell cycle was assessed by flow cytometry (n = 3 biologically independent samples). g, h Measurement of preadipocyte lipid droplet accumulation by Oil Red O staining and spectrophotometric quantification (scale bars: 500 μm, n = 5 biologically independent samples). i The mRNA levels of the preadipocyte differentiation markers PPARG, CEBPA, and AP2 were assessed by RT‒qPCR (n = 6 biologically independent samples). b, d, f, h, i Data were shown as the mean ± SD, p values were obtained using one-way ANOVA followed by Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.

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