Fig. 9: Schematic overview of the study. | Nature Communications

Fig. 9: Schematic overview of the study.

From: Integrative 3D genomics with multi-omics analysis and functional validation of genetic regulatory mechanisms of abdominal fat deposition in chickens

Fig. 9: Schematic overview of the study.

This study successfully established the comprehensive chromatin 3D regulatory network for AFD traits in chickens, integrating WGS, ATAC-seq, histone ChIP-seq, Hi-C, and RNA-seq. The network elaborates on the interactions among regulatory elements, super-regulatory elements, transcription factors, and gene transcription maps. Further, experimental validation of the key variant rs734209466 confirmed that it enhances enhancer activity by interacting with IRF4 and orchestrates the long-range regulation of the IGFBP2 and IGFBP5 genes through loop structures. This modulation promotes adipocyte proliferation and differentiation, thereby intensifying AFD traits. Importantly, this variant does not affect the expression of adjacent genes CXCR1 and TNS1, demonstrating the specificity and precision of the regulatory network.

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