Fig. 3: Single-point mutagenesis of TrkB transmembrane residues. | Nature Communications

Fig. 3: Single-point mutagenesis of TrkB transmembrane residues.

From: Structural basis for the transmembrane signaling and antidepressant-induced activation of the receptor tyrosine kinase TrkB

Fig. 3

a Spatial structure of the TrkBtm dimer, the critical residues of the interface (orange), and the positions of point mutations (green) are indicated. b Amino acid sequence of TrkBtm (highlighted in light blue), the critical residues of the interface (orange), and point mutations (green circles) to Ile (up) or Ala (down) are indicated. c Average length of dendrites upon stimulation of PC12 TrkA-KO cells with BDNF. Data are provided as mean values ± SD. n ≥ 3 biological replicates and n ≥ 27 cells were analyzed (each dot represents one cell). d, e Relative amounts of phosphorylated Y706 and Y707 before and 5 and 15 min after BDNF stimulation of wild-type TrkB and its single-point mutants. Data are provided as mean values ± SD. n ≥ 3 biological replicates (each dot represents one independent replicate). Statistical significance is provided according to the Multiple two-sided t-test with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns denotes that changes with respect to the wt are not significant). Statistical analyses are listed in Supplementary Table 1. Source data are provided as a Source Data file.

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