Fig. 4: Concordant and discordant patterns of H3K27ac-RNA and H3K4me1-RNA correlations suggest divergent regulatory mechanisms in metabolic stress-induced β cell stress and dysfunction.
From: Heterogeneous enhancer states orchestrate β cell responses to metabolic stress
A Scatter plot showing the correlation coefficients of H3K27ac-RNA (y-axis) or H3K4me1-RNA(x-axis) for all genes. Group 1 (green): Genes showing a concordant pattern of H3K27ac-RNA and H3K4me1-RNA correlations. Group 2 (yellow): Genes showing only significant correlations between H3K4me1 and RNA. Group 3 (red): Genes showing only significant correlations between H3K27ac and RNA. Group 4 (grey): Other genes that do not fit into the above correlation patterns. B Pseudo time heatmap of the H3K4me1 gene score (left) and gene expression (right) for all group 1 genes. C Pseudo time heatmap of the H3K27ac gene score (left) and gene expression (right) for all group 1 genes. D Gene ontology for group 1-3 genes showing enrichment in distinct enriched categories. Adjusted p-values were calculated using Benjamini-Hochberg correction. E H3K4me1 (left) and H3K27ac (right) gene scores of representative genes in group 2 (Sik3, Ate1, Sec24d) and group 3 (Dusp10, Kcnmb2) showing the discordant patterns of H3K4me1 and H3K27ac over the β cell dysfunction trajectory. Dots represent gene scores of an individual pseudotime-ordered histone modifications profile. The smoothed line and arrow represent the visualization of the trajectory path from the spline fit. F Heatmap showing the correlation between TF expression and motif activity (left: H3K4me1, right: H3K27ac). G Pie chart showing the H3K27ac-coupled genes (group 1 and 3 from A) that are significantly suppressed by CBP inhibitor GNE-781 or P300 inhibitor C646 (red), or induced by HDACs inhibitor SAHA (green), in primary mouse islets treated with SAHA (5 uM), GNE-781 (1 uM) or C646 (5 uM) for 24 h. H Gene ontology categories of 414 overlapped genes in G.
