Fig. 6: Overexpression of AtHIR4 enhances plant resistance.

a Virulence assay of S. sclerotiorum WT on Col-0 and AtHIR4 overexpression transgenic Arabidopsis lines at 40 hpi. b Lesion area by the cross-over method in (a), n = 10 biologically independent samples. c Relative biomass in (a) analyzed by RT-qPCR with equal area samples from infected sites used for DNA extraction (n = 3 biologically repetitions). d Virulence assay of B. cinerea WT on Col-0 and AtHIR4 overexpression transgenic Arabidopsis lines at 48 hpi. e Lesion areas by the cross-over method in (d), n = 8 biologically independent samples. f Relative biomass in (d) analyzed by RT-qPCR with equal area samples from infected sites used for DNA extraction (n = 3 biologically repetitions). g chitin-induced MAPK activation was significantly enhanced in AtHIR4 overexpression lines. h chitin-induced ROS burst was increased in AtHIR4 overexpression lines, values represent means ± SE (n = 12 biologically repetitions). i Virulence assay of S. sclerotiorum WT on AtHIR4 transgenic rapeseed plants, empty vector transgenic rapeseed plants, and WT rapeseed plants (Y127) at 48 hpi. j Lesion areas by the cross-over method in (I) (n ≥ 10 biologically repetitions). k Relative biomass in (I) analyzed by qPCR with equal area samples from infected sites used for DNA extraction (n = 3 biologically repetitions). l Virulence assay of B. cinerea WT on AtHIR4 transgenic rapeseed plants, empty vector transgenic rapeseed plants, and WT rapeseed plants (Y127) at 48 hpi. m Lesion area by the cross-over method in (l), n = 6 biologically independent samples. n Relative biomass in (l) analyzed by qPCR with equal area samples from infected sites used for DNA extraction (n = 3 biologically repetitions). Data represent means ± SD except for (h). Different letters on the same graph indicate statistical significance at p < 0.01 in one-way ANOVA. Source data are provided as a Source Data file.