Table 1 Content of the 30-plex antibody panel built for protein profiling in developing human lung tissue
From: High-parametric protein maps reveal the spatial organization in early-developing human lung
Antibody-barcode | Targeted protein | In-house conjugation | Targeted main cell type/state | |
|---|---|---|---|---|
1 | CD3-BX015 | CD3D, CD3E, CD3G | No | Immune (T-cell, Innate lymphoid cell) |
2 | CD4-BX021 | CD4 | No | Immune (T-cell) |
3 | CD19-BX003 | CD19 | No | Immune (B-cell) |
4 | CD31-BX032 | CD31/PECAM1 | No | Endothelial |
5 | CD34-BX035 | CD34 | No | Endothelial |
6 | CD45-BX001 | CD45/PTPRC | No | Immune |
7 | CD90-BX022 | CD90/THY1 | No | Mesenchymal (Fibroblast) |
8 | Podoplanin-BX023 | PDPN | No | Lymphatic endothelial, mesothelial |
9 | HLA-DR-BX026 | HLA-DRA, HLA-DRB1,… | No | Immune (Antigen presenting cell) |
10 | Ki67-BX047 | MKI67 | No | Proliferation |
11 | Pan-Cytokeratin-BX019 | KRT8, KRT18,… | No | Epithelial |
12 | E-Cadherin-BX014 | CDH1 | No | Epithelial |
13 | EPCAM-BX042 | EPCAM | Yes | Epithelial |
14 | CD123-BX054 | CD123/IL3RA | Yes | Immune & Endothelial |
15 | SOX2-BX024 | SOX2 | Yes | Epithelial |
16 | CD144-BX016 | CD144/CDH5 | Yes | Endothelial |
17 | ACTA2-BX028 | ACTA2 | Yes | Mesenchymal (Smooth muscle) |
18 | CD68-BX010 | CD68 | Yes | Immune (Macrophage) |
19 | CD44-BX020 | CD44 | Yes | Immune |
20 | DCN-BX013 | DCN | Yes | Mesenchymal |
21 | WT1-BX006 | WT1 | Yes | Mesenchymal |
22 | CD163- BX005 | CD163 | Yes | Immune (Macrophage) |
23 | COL1A1-BX002 | COL1A1 | Yes | Mesenchymal |
24 | CD56-RX029 | CD56/NCAM1 | Yes | Immune (NK cell) |
25 | MRC1-BX030 | MRC1 | Yes | Immune (Macrophage) |
26 | Vimentin-BX045 | VIM | Yes | Mesenchymal |
27 | PRX-BX052 | PRX | Yes | Endothelial |
28 | CLDN5-BX041 | CLDN5 | Yes | Endothelial |
29 | SOX9-BX033 | SOX9 | Yes | Epithelial |
30 | TTF1-BX007 | TTF1/NKX2-1 | Yes | Epithelial |
