Fig. 5: DCR28 genes intermingle with Sc26c38 repeats within the rye B chromosome drive control region.
From: The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly
a Mitotic metaphase of cultivated rye with four standard B chromosomes showing DCR28-specific (magenta) and D1100-specific (green) FISH signals. Bar = 10 μm. b Pachytene chromosomes of wheat with three rye Bs chromosomes showing DCR28-specific (magenta) and Sc26c38-specific (orange) FISH signals. Inlets are showing further enlarged B chromosome regions after FISH. Bar = 10 μm. The FISH experiments were independently repeated twice with similar results. c Multiple copies of DCR28 form a cluster in a 2.3-Mb region. The drive control region (383.9–423.8 Mb) on the chrB was visualized by pyGenomeTracks. The first track (gray) indicates the expression of the RNA-seq data of anthers undergoing the first pollen mitosis (PMI) of wheat with 2Bs. The second track (magenta) represents the DCR28 gene cluster, from 407,247,670 to 409,643,611 bp on chrB. The third track (blue) represents other candidate genes in this region, including three single-copy genes DCR83, DCR260, DCR400, and the DCR169 gene family (dashed box). The fourth track represents other genes in this region that do not belong to the differentially expressed candidates. The fifth track (orange) represents the distribution of the repeat Sc26c38, and the sixth track (green) represents the distribution of the repeat D1100. The last track (sky blue) represents the distribution of the repeat E3900.
