Table 1 Quantitative analysis of the rye B chromosome drive

From: The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly

B varianta

Totalb

No B-specific signalc

Only in VNd

Only in SNe

In all nucleif

Drive?

Drive frequencyg

1 Bk−1

195

103

0

0

92

No

0%

2 Bk−2

77

15

3

55

4

Yes

88.7%

2 Bk−3

101

20

0

0

81

No

0%

2 Bs

112

11

6

91

4

Yes

90.1%

2 Bk

122

21

6

92

3

Yes

91.1%

  1. Wheat lines with different B variants were analyzed by pollen-FISH using the B-specific probe D1100. Refer to Fig. 1 for representative examples of pollen with and without B chromosome drive.
  2. VN vegetative nucleus, SN sperm nuclei.
  3. aWheat possessing different rye B chromosome variants and B numbers.
  4. bTotal number of analyzed pollen.
  5. cNumber of pollen without B-specific signals. Because of meiotic/post-meiotic loss of Bs, not all pollen showed B-specific FISH signals.
  6. dAfter B chromosome nondisjunction, B chromatids enter only the vegetative nucleus (PMI).
  7. eAfter B chromosome nondisjunction, B chromatids enter only the generated nucleus (PMI) and subsequently only sperm nuclei (PMII).
  8. fB chromatids separate normally (disjoin) during PMI and consequently distribute evenly in all nuclei.
  9. gFrequency of pollen with nondisjunction toward generative nucleus among pollen nuclei with B-signals.
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