Fig. 1: Disruption of gene expression upon acute degradation of DDX10 in mESCs. | Nature Communications

Fig. 1: Disruption of gene expression upon acute degradation of DDX10 in mESCs.

From: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells

Fig. 1: Disruption of gene expression upon acute degradation of DDX10 in mESCs.The alternative text for this image may have been generated using AI.

a RT-qPCR analysis for endogenous levels of Ddx10 mRNA in MEFs and mESCs. b RT-qPCR analysis of Ddx10 expression levels following LIF withdrawal. c RT-qPCR analysis of Ddx10 expression levels during embryoid body (EB) differentiation. d Schematic illustration of the generation of DDX10-AID mESCs. e Western blot analysis of DDX10 protein levels in DDX10-AID cells with or without IAA treatment. WT: wild-type E14 mESCs. The red asterisk indicates endogenous DDX10-AID-eGFP and DDX10 proteins. β-ACTIN serves as the loading control. Experiments were repeated three times independently with similar results. f Brightfield images of DDX10-AID (+ OsTir1) mESC colonies with or without IAA treatment. Experiments were repeated three times independently with similar results. Scale bar, 200 µm. g Principal component analysis (PCA) plot displays RNA-seq data from DDX10-AID (+ OsTir1) mESCs treated with IAA at different time points or treated with IAA for 48 h followed by 48 h of washing. h Bar plots showing the number of differentially expressed genes (DEGs) upon DDX10 degradation at different time points with IAA treatment (orange: upregulated genes, green: downregulated genes). i Line chart illustrating gene expression patterns of 24 different clusters of DEGs. j Heatmap presenting gene ontology results for genes in each cluster. For (a–c) transcription levels were normalized against Gapdh. Data are presented as mean values ± SD with the indicated significance from two-sided t-test. Exact p-values are reported in the figure. n = 3 independent experiments. Source data are provided as a Source Data file.

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