Fig. 4: DDX10 regulates the release of U3 snoRNA from pre-rRNA. | Nature Communications

Fig. 4: DDX10 regulates the release of U3 snoRNA from pre-rRNA.

From: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells

Fig. 4: DDX10 regulates the release of U3 snoRNA from pre-rRNA.The alternative text for this image may have been generated using AI.

a Co-IP experiments confirming interactions between DDX10 and BMS1, UTP3, or Fibrillarin in FLAG-tagged DDX10 mESCs. Experiments were repeated three times independently with similar results. b Cartoon diagram illustrating binding sites among U3 snoRNA (magenta), 5’ETS (black), and 18S rRNA (blue). c RIP followed by Northern blot analyzing the interaction between DDX10 and U3 snoRNA. Experiments were repeated three times independently with similar results. d UV absorbance profiles of nuclear extracts fractionated on a 10 – 30% sucrose gradient. The peaks corresponding to 30S and 50S contain small and large ribosomal subunits, respectively, along with their precursors. Earlier preribosomal particles are shown in fractions ahead of 50S peak. e Northern blot showing the distribution of U3 and U14 snoRNAs in individual fractions of DDX10-AID (+ OsTir1) mESCs at 0 h and 48 h after IAA treatment. The data were plotted as the percentage of snoRNAs in each fraction relative to the total in all fractions. Experiments were repeated three times independently with similar results. f Northern blot analysis of the distribution of U3 snoRNA in the preribosome fraction of DDX10-AID (+ OsTir1) mESCs overexpressing DDX10FL, DDX10ΔHBD, and DDX10ΔHCD, respectively, at 0 h and 48 h after IAA treatment. Experiments were repeated two times independently with similar results. Source data are provided as a Source Data file.

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