Fig. 2: AVK photoactivation accelerates locomotion, which is suppressed by AVK-derived FLP-1.

a–f. Wild type and flp-1(ok2811) mutant animals expressing Chrimson specifically in AVK were cultured on NGM plates supplemented with ATR and subjected to behavioral analysis with continuous red light illuminated from t = 900 to 1020 to activate Chrimson. Basal values are the mean from t = 780 to 899. ‘Speed increase’ (c) and ‘Change in body bending’ (f) indicate mean values from t = 900 to 1020 subtracted by basal values. Two-tailed Welch test was performed. ***p < 0.001. Approximately 20–120 animals were involved in each recording. Part of measurements are overlapping with Supplementary Fig. 3e. g–l. Wild type and flp-1 mutant RNAi strains expressing Chrimson in AVK were cultivated with HT115 bacteria carrying a control vector or a plasmid producing dsRNA for flp-1 with ATR for 3 days and subjected to behavioral analysis. Dunnett test was performed against wild type animals fed with control HT115 bacteria. ***p < 0.001. Approximately 25–120 animals were involved in each recording. In timeseries plots, data are presented as mean values +/- SEM. In boxplots, the boxes extend from the Q1 to Q3, with the band inside the boxes representing the median. The whiskers extend to the smallest and largest values within 1.5 times the IQR. Source data are provided in ‘Source Data 1’ file.